Section 1



Signal Transduction and Gene Regulation in the Hypothalamic-Pituitary-Gonadal Axis (Stephen Hammes, Section Director)

Section 1 emphasizes signal transduction and gene regulation in the hypothalamic-pituitary-gonadal axis. Lectures cover physiologic to molecular actions of peptide, protein and steroid hormones and the intracellular signaling as well as the transcriptional gene regulatory mechanisms that drive reproductive biology. Section 1 laboratories teach methods key to studying hormone action and cell biology. Techniques include quantification of second messengers and transcriptional activity; cell culture and transient transfection; western blot analysis; ELISAs; quantitative RT-PCR; gene silencing; chromatin immunoprecipitation; immunofluorescence and in situ hybridization; mutagenesis; tissue dissection. Principles of microscopy and imaging methods are also discussed and applied.

The schedule for Section 1 of FIR2020 can be downloaded here.