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Frontiers in Reproduction 2008
Section 1 Schedule as of 4/8/08
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Sunday May 4
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Welcoming Reception at 6 PM
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Monday May 5
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Lectures
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Lab
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9 AM. Jon Levine Feedback, Fetal Development, and Fertility:
Neuroendocrine Regulation of
Ovulatory Cycles |
Mario Ascoli Pathways Activated via LHR
Into: Signaling pathways activated by the wild-type and by constitutively active mutants of the lutropin receptor (PDF)
Experiment #1: Cyclic AMP and progesterone accumulation in MA-10 cells (PDF)
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| 1 PM. Jim Marcello. Lab Safety at the MBL |
7 PM. Kelly Mayo Introduction to Cellular and Molecular Endocrinology I: Gene Expression and its Analysis
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Tuesday May 6
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Lectures
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Lab
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| 9 AM. Mario Ascoli Signal Transduction in the hypothalamic-pituitary-gonadal axis |
Mario Ascoli Pathways Activated via LHR
Experiment #2: Inositol phosphate accumulation in MA-10 cells (PDF)
Experiment #3: Binding of 125I-hCG to MA-10 cells (PDF)
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| 7 PM. Leslie Heckert Signaling to the nucleus |
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Wednesday May 7
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Lectures
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Lab
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| 9 AM. Ursula Kaiser Differential Regulation of Gonadotropin Genes: Endocrine Factors, Paracrine Factors, and Transcription Factors |
Mario Ascoli Pathways activated via LHR
Experiment #4: Phosphorylation of CREB and ERK1/2 in MA-10 cells (PDF)
Protein Assay for Cell Lysates Adapted from BCA assay kit of Bio Rad (PDF) |
7 PM. Mario Ascoli New insights into the signaling pathways activated by the lutropin receptor in Leydig
cells.
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Thursday May 8
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Lectures
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Lab
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| 9 AM. Sally Camper PITX2 in pituitary development: Genetically engineered mice reveal that transcription factors have dosage-sensitive effects and distinct roles at different developmental stages |
Mario Ascoli Pathways activated via LHR
Experiment # 5: Transfection of MA-10 cells and binding assay (PDF) |
| 7 PM. Blanche Capel The Battle of the Sexes: Antagonistic Signals Regulate Sex Determination |
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Friday May 9
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Lectures
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Lab
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| 9 AM. Ken Korach Consequences in Reproduction from the Loss of Estrogen Receptor Activity |
Ruth Keri Web-based bioinformatics tools for molecular endocrinology |
| 7 PM. Mario Ascoli Lab wrap-up/pizza party |
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Saturday May 10
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Lectures
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Lab
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9 AM. Ruth Keri Using functional genomics to identify mechanisms of breast carcinogenesis
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Leslie Heckert & Brian Hermann Transcription Lab |
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Sunday May 11
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Free Day
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Monday May 12
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Lectures
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Lab
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| 9 AM. Leslie Heckert Steroidogenic factor 1 and the regulation of Leydig cell development |
Leslie Heckert & Brian Hermann Transcription Lab
Heckert FIR Protocols (PDF)
Heckert Solution Recipes (Excel)
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| 7 PM. Bob Braun AR Signaling in Germ Cell Development |
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Tuesday May 13
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Lectures
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Lab
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| 9 AM. Barry Zirkin Regulation of Leydig Cell Function |
Leslie Heckert & Brian Hermann Transcription Lab |
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| 7 PM. Don DeFranco Nuclear Receptors and Coactivators |
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Wednesday May 14
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Lectures
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Lab
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| 9 AM. Don DeFranco Analysis of a tissue compartment-specific nuclear receptor coactivator |
Leslie Heckert & Brian Hermann Transcription Lab |
| 7 PM. Marco Conti LH signaling and the EGF network in the ovarian follicle |
Don DeFranco and Michelle Wood. Nuclear Receptors
DeFranco Protocols: Steroid Receptor-Dependent Gene Transcription (PDF)
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Thursday May 15
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Lectures
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Lab
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| 9 AM. Bruce Murphy Role of the nuclear receptor NR5A2 (liver receptor homolog-1) in follicle development |
Don DeFranco and Michelle Wood. Nuclear Receptors |
| 7 PM. Open (TBA) |
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Friday May 16
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Lectures
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Lab
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| 9 AM. Open (TBA) |
Don DeFranco and Michelle Wood. Nuclear Receptors |
| 7 PM. DeFranco Lab wrap-up/pizza party |
| TO FOLLOW: Leslie Heckert Section wrap-up |
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Saturday May 17
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Free Day
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